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Enzymatic Conversion of the Stereoisomers of isoLeucine to the Corresponding α-Keto-Acids

✍ Scribed by MEISTER, ALTON


Book ID
109567776
Publisher
Nature Publishing Group
Year
1951
Tongue
English
Weight
113 KB
Volume
168
Category
Article
ISSN
0028-0836

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A spectrophotometric endpoint assay for determination of branched-chain cu-keto acids is described. The assay depends on measurement of the NADH produced after addition of branched-chain cY-keto acid dehydrogenase. Interference by pyruvate and cy-ketobutyrate was eliminated by pretreating the sample