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Enzymatic assay of myo-inositol in serum

โœ Scribed by Roswitha Dolhofer; O. H. Wieland

Book ID
112334832
Publisher
Springer
Year
1986
Tongue
English
Weight
123 KB
Volume
324
Category
Article
ISSN
1618-2650

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An enzymatic assay for my&nositol (MI) is described. The method is based on the oxidation of MI by NAD+dependent my&nositol dehydrogenase, coupled to reoxidation of NADH with oxalacetate and malate dehydrogenase. The resultant malate is measured fluorimetrically. Several variations of the assay are

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The determination of my@inositol t&phosphate by an enzymatic fluorometric assay is presented. The method involves the acid extraction of water-soluble inositol polyphosphates followed by separation by anion-exchange chromatography. Samples are subsequently neutralized by passage over a Dowex c1-resi