Enzymatic Assay of Galactosyltransferase by Capillary Electrophoresis
โ Scribed by Yoshimi Kanie; Annette Kirsch; Osamu Kanie; Chi-Huey Wong
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 125 KB
- Volume
- 263
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
Galactosyltransferase catalyzes transfer of galactose from UDP-galactose to glucose or N-acetylglucosamine with resultant formation of galactosides and UDP. In this new assay galactosyltransferase activity is measured by determining UDP by isocratic high-performance liquid chromatography on an amino
Using capillary zone electrophoresis, the urine creatinine (uCr) assay was validated in extemporaneous diluted urine, both in healthy subjects and athletes, with the uCr concentration as a reference value to compare excretion rates of other metabolites in the same samples. The electrokinetic sample
\(\beta\)-Galactosidase can be assayed by monitoring the generation of the fluorescent products, fluoresceinmono- \(\beta\)-D-galactopyranoside and fluorescein, when the fluorogenic substrate fluorescein-di- \(\beta\)-D-galactopyranoside is used. We have used capillary electrophoresis with ultrasens