A spectrophotometric endpoint assay for determination of branched-chain cu-keto acids is described. The assay depends on measurement of the NADH produced after addition of branched-chain cY-keto acid dehydrogenase. Interference by pyruvate and cy-ketobutyrate was eliminated by pretreating the sample
Enzymatic and pharmacokinetic studies on the metabolism of branched chain α-keto acids in the rat
✍ Scribed by Bässler, K. H. ;Pietrek, Astrid
- Publisher
- Springer
- Year
- 1983
- Tongue
- English
- Weight
- 757 KB
- Volume
- 22
- Category
- Article
- ISSN
- 1436-6215
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
## Abstract Leucine aminotransferase (EC 2.6.1.6) and 2‐oxoisocaproate dehydrogenase (EC 1.2.4.3) were studied in rat cerebral cortex, cerebellum, brain stem, liver, and muscle in normal and animals starved for 48 hours. In the brain, leucine aminotransferase, valine aminotransferase, and 2‐oxoisoc
The aim of this study was to investigate the effect of oral administration of branched-chain amino acid (BCAA)-enriched diets after portacaval shunt (PCS) in rats. Fifty-one Sprague-Dawley male rats (200 gm) underwent PCS and 55 a sham operation. Half of the animals received BCAA 142 mg per day thro