Enzymatic activites during differentiation of the human neuroblastoma cells, LA-N-1 and LA-N-2

✍ Scribed by I. N. Singh; G. Sorrentino; D. G. McCartney; R. Massarelli; Professor J. N. Kanfer

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 824 KB
• Volume: 25
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/jnr.490250405

✦ Synopsis

Abstract

The presence of 10^−5^ M retinoec acid (RA) in the culture medium of LA‐N‐1, a catecholamonergic cell line, and LA‐N‐2, a cholinergic cell line, enhanced their morphological differentiation. Tyrosine hydroxylase (TH) activity of the LA‐N‐1 cell was increased in the RA‐treated cells compared with control cultures at day 4 and remained elevated. Choline actyltransferase (ChAT) activity in the LA‐N‐2 cells gradually increased until 8 days in vitro (DIV) voth in the untreated control and the RA treated cultures. This activity in control and treated cells decreased gradually to a constant level of activity. The ChAT activity at 8 DIV of RA‐treated La‐N‐2 cells was increased 2.1‐fold (P<0.001) as compared to the control cultures. This increase in ChAT activity was accompanied by a 73% decrease of acetylcholinesterase (AChE) activity in LA‐N‐2 cell by 8DIV. AChE activity of LA‐N‐1 cells was unchanged during the time course of the experiment. Phospholipase‐A~2~ (PL‐A~2~)activity in RA‐treated LA‐N‐2cells was increased at day 4 as compared with the control cultures. There were no differnces obseved in phopholipase‐D (PL‐D), choline kinase and FPC‐phosphodiesterases activities in RA‐treated and ‐untreated LA‐N‐1 and LA‐N‐2 cells.