Retinoic acid and methotrexate specifically increase PHA-E-lectin binding to a 67-kDa glycoprotein in LA-N-1 human neuroblastoma cells
✍ Scribed by Sharon A. Ross; Carol S. Jones; Luigi M. De Luca
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- French
- Weight
- 765 KB
- Volume
- 62
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
Laboratory of Cellular Carcinogenesis and Tumor Promotion; and 2Cancer Prevention Fellowship Prograq
Retinoic acid (RA) decreased growth and increiwd morphologic differentiation of human neuroblastoma LA-N-I cells. These phenomena correlated with a specific enhancement of PHA-E lectin binding to a glycoprotein of MW 67 kDa (gp67). Gp67 was found susceptible to N-glycanase and displayed BSA binding by affinity chromatography analysis. The chemotherapeutic agent methotrexate (MTX) also reduced growth and induced differentiation of LA-N-I cells. In addition, the cells responded to MTX as well as to doxorubicin by a marked increase in PHA-E binding to gp67. We conclude that reduced growth and induction of morphological differentiation of LA-N-I cells correlates with increased binding of PHA-E to gp67.