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Enzymatic activation of 5-formyltetrahydrofolate via conversion to 5,10-methenyltetrahydrofolate

โœ Scribed by F.M. Huennekens; G.B. Henderson; K.S. Vitols; C.E. Grimshaw


Book ID
118262843
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
708 KB
Volume
22
Category
Article
ISSN
0065-2571

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Enzymatic conversion of deoxycytidine 5โ€ฒ
โœ Richard Y.-H. Wang; Lan-Hsiang Huang; Melanie Ehrlich ๐Ÿ“‚ Article ๐Ÿ“… 1982 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 465 KB

An enzymatic method has been developed to synthesize S-methyldeoxycytidine triphosphate (m'dCTP) starting with dCMP and formaldehyde as substrates. Methylation is catalyzed by a dCMP methyltransferase in the presence of tetrahydrofolic acid. The resulting m'dCMP is converted to m'dCTP by a kinase pr