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Enteroviral polymerase chain reaction in the investigation of aseptic meningitis

✍ Scribed by Riding, Miriam H.; Stewart, Jane; Clements, Geoffrey B.; Galbraith, Daniel N.

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 326 KB
Volume: 50
Category: Article
ISSN: 0146-6615
DOI: 10.1002/(sici)1096-9071(199610)50:2<204::aid-jmv16>3.0.co;2-l

No coin nor oath required. For personal study only.

✦ Synopsis

We used a nested polymerase chain reaction (nPCR) to seekevidence for enteroviruses in clinical samples from patients with symptoms of aseptic meningitis. When compared with conventional virus isolation methods on a total of 366 samples collected during 1994-1995, an increase in positivity from 6% to 27% was shown.

The results indicate that nPCR would be a valuable aid to the laboratory diagnosis of enteroviral infections as it can detect those enteroviruses that cannot be identified by current isolation methods. o 1996 Wiley-Liss, Inc.

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