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Entamoeba histolytica: Identification and partial characterization of α-mannosidase activity

✍ Scribed by Clara E. Santacruz-Tinoco; Julio C. Villagómez-Castro; Everardo López-Romero


Book ID
113617926
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
577 KB
Volume
124
Category
Article
ISSN
0014-4894

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Partial characterization of α-mannosidas
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An exo-a-mannosidase (E.C. 3.2.1.24) was characterized in the yeast form of Yarrowia lipolytica. The enzyme located in a crude particulate fraction of the cell extract is under catabolite repression, has an optimum pH of 6.0, a Km of 0.27 m~ with p-nitrophenyl-lx-o-mannopyranoside and is partially i