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Enrichment for auxotrophic and heat-sensitive mutants of Neurospora crassa by tritium suicide

✍ Scribed by Peter J. Russell; Marguerite P. Cohen

Book ID
113190967
Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
427 KB
Volume
34
Category
Article
ISSN
0027-5107

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A heat-sensitive mutant of Neurospora crassa, strain 4M(t), was isolated using ultraviolet-light mutagenesis followed by the inositol-less death enrichment technique. The heat-sensitivity is the result of a single gene mutation which maps to the distal end of the right arm of linkage group II. The m