Enkephalin analogs as substrates for the assay of brain cysteine proteinase (Cathepsin L) and its endogenous inhibitors

The hydrolysis of N-benzoyl-l-phenylalan

The hydrolysis of N-benzoyl-l-phenylalanyl-l-valyl-l-arginine-p-nitroanilide and its use as a substrate for the assay of cathepsin B

✍ John Butterworth; Judith J. Duncan 📂 Article 📅 1980 🏛 Elsevier Science 🌐 English ⚖ 428 KB

A calorimetric assay for the measurement of cathepsin B using N-benzoyl-r.-phenylalanyl-L-valyl+arginine-p-nitroanilide (S2160) as substrate has been developed. The technique is reproducible and simpler, quicker, safer, and more sensitive than that using cr-N-benzoyl-DL-arginine-P-naphthylamide as s