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Enhancing the analytical performance of immunoassays that employ metal-enhanced fluorescence

✍ Scribed by Robert Nooney; Andrew Clifford; Xavier LeGuevel; Ondrej Stranik; Colette McDonagh; Brian D. MacCraith


Book ID
105892901
Publisher
Springer
Year
2009
Tongue
English
Weight
283 KB
Volume
396
Category
Article
ISSN
1618-2650

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## Abstract Nucleic acid can greatly enhance the fluorescence intensity of quercetin in HMTA‐HCl (pH 5.5) buffer. The enhanced intensity is in proportion to the concentration of nucleic acids in the range 5.0Β Γ—Β 10^βˆ’9^ to 1.0Β Γ—Β 10^βˆ’6^g/mL for fsDNA, 5.0Β Γ—Β 10^βˆ’9^ to 7.0Β Γ—Β 10^βˆ’7^g/mL for ctDNA and 5.0