Enhancing protein solubilization with nondetergent sulfobetaines
β Scribed by Dr. Laurent Vuillard; Nathalie Marret; Thierry Rabilloud
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 335 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A homologous series of novel zwitterionic detergents, sulfobetaines (SB,), was examined for its ability to emulsify a triglyceride model system and to extract proteins from 3T6 mouse fibroblast membranes. In both instances the solubilization efficiency of SB,s was found to improve with increasing al
## Abstract A protein solubilization method has been developed to directly solubilize protein clusters into organic solvents containing small quantities of surfactant and trace amounts of water. Termed βdirect solubilization,β this technique was shown to solubilize three distinct proteinsβsubtilisi