✦ LIBER ✦

Enhancing multiple disulfide bonded protein folding in a cell-free system

✍ Scribed by Gang Yin; James R. Swartz

Publisher: John Wiley and Sons
Year: 2004
Tongue: English
Weight: 553 KB
Volume: 86
Category: Article
ISSN: 0006-3592
DOI: 10.1002/bit.10827

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Protein disulfide isomerase, but not bin

Protein disulfide isomerase, but not binding protein, overexpression enhances secretion of a non-disulfide-bonded protein in yeast

✍ Jason D. Smith; Benjamin C. Tang; Anne Skaja Robinson 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 532 KB

Protein phosphorylation in a cell-free t

Protein phosphorylation in a cell-free translation system from Vicia sativa

✍ F.J. Arias; M.A. Rojo; J.M. Ferreras; R. Iglesias; R. Muñoz; T. Girbést 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 265 KB

Rapid expression of vaccine proteins for

Rapid expression of vaccine proteins for B-cell lymphoma in a cell-free system

✍ Junhao Yang; Gregory Kanter; Alexei Voloshin; Nathalie Michel-Reydellet; Hendrik 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 389 KB

Simultaneous expression and maturation o

Simultaneous expression and maturation of the iron-sulfur protein ferredoxin in a cell-free system

✍ Marcus E. Boyer; Chia-Wei Wang; James R. Swartz 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 245 KB 👁 2 views

## Abstract The model iron‐sulfur (Fe‐S) protein ferredoxin (Fd) from __Synechocystis sp__. PCC 6803 has been simultaneously produced and matured in a cell‐free production system. After 6 h of incubation at 37°C, Fd accumulated to >450 µg/mL. Essentially all was soluble, and 85% was active. Product

Tuning the expression level of recombina

Tuning the expression level of recombinant proteins by modulating mRNA stability in a cell-free protein synthesis system

✍ Jin-Ho Ahn; Taek-Jin Kang; Dong-Myung Kim 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 253 KB 👁 2 views

## Abstract In this work, it was discovered that the stability of mRNA in a cell‐free extract could be controlled by using engineered T7 terminator sequences. Specifically, it was found that mRNA stability gradually decreased as the length of the stem structure of the T7 terminator was reduced sequ

Combinatorial, selective and reversible

Combinatorial, selective and reversible control of gene expression using oligodeoxynucleotides in a cell-free protein synthesis system

✍ Jung-Won Keum; Jin-Ho Ahn; Taek Jin Kang; Dong-Myung Kim 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 254 KB 👁 2 views

## Abstract Herein we describe the methods for selective and reversible regulation of gene expression using antisense oligodeoxynucleotides (ODNs) in a cell‐free protein synthesis system programmed with multiple DNAs. Either a complete shut down or controlled level of gene expression was attained t