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Enhancement of plasminogen activator activity in cultured endothelial cells by granulocyte colony-stimulating factor

✍ Scribed by Soichi Kojima; Hirohiko Tadenuma; Yuji Inada; Yuji Saito

Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
533 KB
Volume
138
Category
Article
ISSN
0021-9541

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✦ Synopsis

A hitherto unknown function of granulocyte colony-stimulating factor (G-CSF) was found using cultured endothelial cells. G-CSF stimulated activity of plasminogen activator (PA) in both extracellular and intracellular milieus of endothelial cells obtained from bovine carotid and aortic artery. This effect was dependent on the concentration of G-CSF added to the culture medium and on the treatment time. The extracellular activity was enhanced approximately 5-fold at a concentration of 5,000 colony-forming unit (CFU)/mI (2.6 nM) and in about a 15-hr treatment period. Analyses by fibrin and reverse fibrin autography revealed that activity of PA was much more increased than that of PA inhibitor in endothelial cells treated with G-CSF.

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