Enhanced human lymphokine-activated killer cell function after brief exposure to granulocyte-macrophage–colony stimulating factor
✍ Scribed by Constantin N. Baxevanis; George V. Z. Dedoussis; Nikolaos G. Papadopoulos; Ioannis Missitzis; Constantin Beroukas; George P. Stathopoulos; Michael Papamichail
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 833 KB
- Volume
- 76
- Category
- Article
- ISSN
- 0008-543X
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✦ Synopsis
Background. Lymphokine-activated killer (LAK) cell function can be generated in peripheral blood mononuclear cells (PBMC) after brief exposure of high dose interleukin-2 (IL-2) over the course of 1 or 2 days' culture in plain culture medium (IL-%pulsed PBMC). The aim of the present study was to investigate the ability of granulocyte-macrophage-colony stimulating factor (GM-CSF) to augment LAK induction in low dose IL-%pulsed PBMC derived from patients with cancer undergoing immunotherapy with IL-2.
Methods. Peripheral blood mononuclear cells were collected from patients with cancer receiving a 5-day cycle of local (intraperitoneal or intrapleural) infusions with IL-2. The cells were incubated with IL-2 in the presence or absence of GM-CSF for 1 hour and then tested as effectors against allogeneic tumor cells and LAK-sensitive cell lines.
Results. Granulocyte-macrophage-colony stimulating factor at doses between 10 and 100 ng/ml was synergized with low dose IL-2 (100 IU/ml) in the generation of LAK activity in PBMC. Lymphokine-activated killer cellmediated cytotoxicity derived from PBMC cultures incuhated with IL-2 and GM-CSF was significantly higher (up to three-fold) compared with that generated with IL-2 alone. The GM-CSF-induced enhanced LAK activity was maintained when tested at day 5. GM-CSF increased the percentages of IL-2 receptor (R) positive (' ) and CD8' cells in the IL-&pulsed PBMC. In contrast to CD56f cells,