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Enhancement of carbohydrates in a methylotrophic yeast

✍ Scribed by Gene R. Petersen; Barry O. Stokes; Wayne W. Schubert; Angela M. Rodriguez


Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
481 KB
Volume
5
Category
Article
ISSN
0141-0229

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We developed a host-vector system for transformation and gene cloning experiments using the methylotrophic yeast Hansenula p o l y w p h a . Regeneration of protoplasts in a medium containing polyethylene glycol before plating made transformation more efficient and reproducible (2 to 3 -lo4 pg DNA).