Enhancement of berberine production by spermidine inThalictrum minuscell suspension cultures
โ Scribed by Masakazu Hara; Yoshinori Kobayashi; Hiroshi Fukui; Mamoru Tabata
- Publisher
- Springer
- Year
- 1991
- Tongue
- English
- Weight
- 301 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0721-7714
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โฆ Synopsis
Berberine production in cell suspension cultures of Thalictrum minus L. var. hypoleucum Miq. was significantly enhanced by administration of spermidine, whereas other polyamines such as cadaverine, putrescine and spermine were ineffective. The results of experiments indicated that spermidine causes an increase of ethylene generation which is closely associated with activation of berberine biosynthesis.
๐ SIMILAR VOLUMES
Cultured cells of Thalictrum minus L. (Ranunculaceae), transferred from culture flasks to a bubble column bioreactor, produced little berberine and turned dark brown, even when supplied with sufficient oxygen. This phenomenon was ascribed to the removal of CO2 from the culture medium by bubbling air
## Abstract Azadirachtin is considered as the best pesticide among the biopesticides available in market for crop protection due to its broad spectrum activity and varying mode of action. To meet the increasing demand and primarily due to geographical and variability limitations, its biotechnologic