Abstract
The present study aimed to investigate the effect of morin on the pharmacokinetics of methotrexate (MTX) in rats. Pharmacokinetic parameters of MTX were determined in rats following an intravenous administration of MTX (2βmg/kg) in the presence and absence of morin (25βmg/kg, p.o.). The cellular accumulation of MTX was also examined by using MDCK cells stably overexpressing hOAT1 or hOAT3. Compared with the control given MTX alone, pretreatment with morin 15 min prior to MTX administration significantly altered the pharmacokinetics of MTX in rats. Renal clearance and total clearance of MTX were reduced by 42% and 58%, respectively, in the presence of morin. Accordingly, the systemic exposure of MTX in the rats pretreated with morin was significantly higher than that from the control group. The mean residence time (MRT) and terminal plasma halfβlife of MTX were prolonged by 3.3β and 2.4βfold, respectively, by the concurrent use of morin. The cellular uptake of MTX (20βΒ΅M) was significantly reduced by the coβincubation with morin (100βΒ΅M) in MDCKβhOAT1 cells but not in MDCKβhOAT3 cells. Taken together, morin appeared to be effective in altering the pharmacokinetics of MTX in rats, likely by the inhibition of OAT1βmediated renal excretion. Copyright Β© 2008 John Wiley & Sons, Ltd.