Enhanced selectivity in flow-injection analysis for l-amino acids using electrodialysis with amino acid oxidation

✍ Scribed by J.C. Cooper; J. Danzer; H.-L. Schmidt

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 906 KB
• Volume: 282
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)80223-8

✦ Synopsis

A method has been developed for continuous determination of the concentration of one ammo acid in a mixture, using electrodialysis combined with a flow-injection system. Using a non-specific ~-amino acid oxidase column and electrochemical detection of enzymatically produced hydrogen peroxide, the concentration of a particular amino acid in a mixture could be determined by first performing a separation step using an electrodialysis unit. Using the separation of arginine as a model system, it was possible to determine the concentration of this amino acid in the presence of two or three of a group of nine amino acids.