## Abstract __Pseudomonas putida__ KT2442 could accumulate medium‐chain‐length poly(hydroxyalkanoate)s (PHA) consisting of 3‐hydroxyhexanoate, 3‐hydroxyoctanoate, 3‐hydroxydecanoate, and 3‐hydroxydodecanoate from a wide range of carbon sources. In this study, the PHA synthase __pha__ operon (phaC1‐
Enhanced production of medium-chain-length polyhydroxyalkanoates (PHA) by PHA depolymerase knockout mutant of Pseudomonas putida KT2442
✍ Scribed by Lei Cai; Mei-Qing Yuan; Feng Liu; Jia Jian; Guo-Qiang Chen
- Publisher
- Elsevier Science
- Year
- 2009
- Tongue
- English
- Weight
- 489 KB
- Volume
- 100
- Category
- Article
- ISSN
- 0960-8524
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High-cell-density fed-batch cultures of Pseudomonas putida were carried out for the production of me- dium-chain-length polyhydroxyalkanoates (PHAs) using oleic acid as a carbon source. By employing an optimal feeding strategy without the limitation of any nutrient, a high cell concentration of 173
Pseudomonas putida KT2442 is able to accumulate medium-chain-length poly(3-hydroxyalkanoates) (mcl-PHAs) as intracellular inclusions on a variety of fatty acids and many other carbon sources. Some of these substrates, such as octanoic acid, alkenoic acids, and halogenated derivatives, are toxic when