Production of medium-chain-length polyhydroxyalkanoates by high-cell-density cultivation of Pseudomonas putida under phosphorus limitation

✍ Scribed by Sang Yup Lee; Heng Ho Wong; Jong-il Choi; Seung Hwan Lee; Sang Cheol Lee; Chul Soo Han

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 131 KB
• Volume: 68
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/(sici)1097-0290(20000520)68:4<466::aid-bit12>3.0.co;2-t

✦ Synopsis

High-cell-density fed-batch cultures of Pseudomonas putida were carried out for the production of me- dium-chain-length polyhydroxyalkanoates (PHAs) using oleic acid as a carbon source. By employing an optimal feeding strategy without the limitation of any nutrient, a high cell concentration of 173 g/L was achieved, but the PHA concentration and PHA content were only 32.3 g/L and 18.7 wt%, respectively. To increase the PHA concentration and content, phosphorus limitation was applied during fed-bath culture by reducing the initial KH 2 PO 4 concentration. When the initial KH 2 PO 4 concentration was reduced to 4 g/L, cell concentration, PHA concentration, and PHA content obtained in 38 h were 141 g/L, 72.6 g/L, and 51.4 wt%, respectively, resulting in a high productivity of 1.91 g PHA/L per hour.