𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Enhanced phosphoinositide metabolism in colorectal carcinoma cells derived from familial adenomatous polyposis patients

✍ Scribed by Miwako K. Homma; Yoshimi Homma; Masayoshi Namba; Yasuhito Yuasa

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
870 KB
Volume
55
Category
Article
ISSN
0730-2312

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The production of the second messenger molecules diacylglycerol and inositol 1,4,5‐trisphosphate is mediated by activated phosphatidylinositol‐specific phospholipase C (PLC) enzymes. We report the enhancement of the phosphoinositide metabolism pathway in KMS‐4 and KMS‐8 cells, both of which are human colorectal carcinoma cell lines derived from familial adenomatous polyposis patients. In these cells, the cellular contents of diacylglycerol and inositol 1,4,5‐trisphosphate were constitutively increased and the PLC activity in vitro was significantly high, as compared with those in normal colon cells or in other sporadic colorectal carcinoma cells. Northern and Western analyses showed the high expression levels of both PLC‐γ1 and PLC‐δ1 in KMS‐4 and KMS‐8 cells. Moreover, we detected the enhancement of protein–tyrosine kinase activity and tyrosine phosphorylation of PLC‐γ1 in these KMS cells. These results suggest the involvement of activated phosphoinositide signaling pathways in the colorectal tumorigenesis of familial adenomatous polyposis. © 1994 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Molecular nature of chromosome 5q loss i
Molecular nature of chromosome 5q loss in colorectal tumors and desmoids from patients with familial adenomatous polyposis
✍ Mieko Okamoto; Chieko Sato; Yuko Kohno; Takeo Mori; Takeo Iwama; Akira Tonomura; 📂 Article 📅 1990 🏛 Springer 🌐 English ⚖ 600 KB

Familial adenomatous polyposis (FAP), which includes familial polyposis coli (FPC) and the Gardner syndrome (GS), is a genetically determined premalignant disease of the colon inherited by a locus (APC) mapping within 5q15-q22. To elucidate the role of 5q loss in FAP tumorigenesis, we analysed 51 co

Loss of normal allele of the APC gene in
Loss of normal allele of the APC gene in an adrenocortical carcinoma from a patient with familial adenomatous polyposis
✍ Madoka Seki; Kiyoko Tanaka; Rei Kikuchi-Yanoshita; Motoko Konishi; Hiroyuki Fuku 📂 Article 📅 1992 🏛 Springer 🌐 English ⚖ 272 KB

Endocrine neoplasms have been reported occasionally in patients with familial adenomatous polyposis (FAP). An adrenocorotical carcinoma was studied in a patient with a family history of FAP. Loss of heterozygosity (LOH) in the region close to the adenomatous polyposis coli (APC) gene was detected in

Interstitial loss of the same region of
Interstitial loss of the same region of 5q in multiple adenomas and a carcinoma derived from an adenomatous polyposis coli (apc) patient
✍ Yoshio Miki; Isamu Nishisho; Yusuke Nakamura; Yasuo Miyoshi; Joji Utsunomiya 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 341 KB 👁 2 views

## Abstract Accumulation of genetic alterations in oncogenes and tumor suppressor genes causes the transformation of a normal cell into a malignant cell. Recently, Fearon and Vogelstein (Cell 61:759‐767, 1990) reported on a model for the genetic pathway in development of colorectal neoplasia. To in

Detection in colorectal carcinoma patien
Detection in colorectal carcinoma patients of antibody cytotoxic to established cell strains derived from carcinoma of the human colon and rectum
✍ Richard M. Schultz; Wilna A. Woods; Michael A. Chirigos 📂 Article 📅 1975 🏛 John Wiley and Sons 🌐 French ⚖ 567 KB

## Abstract Sera from eight of 15 patients with colonic carcinoma exhibited demonstrable cytotoxicity against an established cell strain derived from adenocarcinoma of the ileocecum, HCT‐8. Sera from 12 of 16 patients with rectal carcinoma were cytotoxic for an established cell strain derived from