Enhanced intraspecific protoplast fusion in yeast
โ Scribed by K. Kavanagh; M. Walsh; P.A. Whittaker
- Book ID
- 109317227
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 299 KB
- Volume
- 81
- Category
- Article
- ISSN
- 0378-1097
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Fusion of protoplasts prepared from haploid strains of Saccharomyces yeasts having identical mating type was induced with the aid of polyethylene glycol. Stable fusion products were isolated by complementation of the auxotrophic markers. Of 64 isolates derived by protoplast fusion between two differ
The technique of protoplast fusion has been applied to the problem of unstable diploidy in the yeast Kluyveromyces lactis. By protoplast fusion between heterothallic strains of like mating-type, sporulationdeficient hybrids can be obtained. Biochemical, cytological, and genetical characterisation of
The production of ฮฑ-amylase was increased by protoplast fusion of auxotrophic mutants of Candida fennica FTPT-8903. One prototrophic fusant was 90% and 32% more efficient in producing ฮฑ-amylase in semi-solid and liquid fermentation, respectively, than the parental strains. Protoplast fusion did not