Enhanced Fructose Oxidase Activity in a Galactose Oxidase Variant
β Scribed by Sarah E. Deacon; Khaled Mahmoud; R. Kate Spooner; Susan J. Firbank; Peter F. Knowles; Simon E. V. Phillips; Michael J. McPherson
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 179 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1439-4227
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π SIMILAR VOLUMES
The relative orientation of the two magnetic orbitals, the Cu dβxβ2-yβ2 orbital and the half-occupied Ο orbital of the tyrosyl radical, is the key to answering the question in the title. The arrangement shown (Cu -O-C bond angle of about 130Β° and a dihedral angle of about 90Β° between the x,y plane o
## Abstract Galactose oxidase (E.G. 1.1.3.9) was covalently immobilized to chemically modified porous silica particles by reaction of the native enzyme with pendant benzoyl azide groups on the carrier. The enzyme loading on the carrier was 100β150 units per milliliter. The immobilized enzyme was in