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Enhanced expression of heregulin in c-erb B-2 and c-Ha-ras transformed mouse and human mammary epithelial cells

✍ Scribed by G. Mincione; C. Bianco; S. Kannan; G. Colletta; F. Ciardiello; M. Sliwkowski; Y. Yarden; N. Normanno; A. Pramaggiore; N. Kim; D.S. Salomon

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
978 KB
Volume
60
Category
Article
ISSN
0730-2312

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✦ Synopsis

Heregulin P1 was found to stimulate the anchorage-dependent, serum-free growth of nontransformed human MCF-1 OA mammary epithelial cells. Unlike epidermal growth factor, transforming growth factor a, or amphiregulin, heregulin P I was also able to induce the anchorage-independent growth of MCF-1OA cells. In contrast, the anchorage-dependent, serum-free growth of c-Ha-ras or c-erb 6-2 transformed MCF-1 OA cells was unaffected by heregulin PI, whereas heregulin P I was able to stimulate the anchorage-independent growth of these cells. c-Ha-ras or c-erb 8-2 (c-neu) transformed MCF-1 OA or mouse NOG-8 mammary epithelial cells express elevated levels of 2.5, 5.0, 6.5, 6.8, and 8.5 kb heregulin mRNA transcripts and/or synthesize cell-associated 25, 29, 50, and 11 5 kDa isoforms of heregulin. Since the MCF-1 OA cells and transformants also express c-erb 8-3, these data suggest that endogenous heregulin might function as an autocrine growth factor for Ha-ras or erb 6-2 transformed mammary epithelial Cells.

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