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Enhanced enantioselectivity of a carboxyl esterase fromRhodobacter sphaeroidesby directed evolution

✍ Scribed by Ma, Jingbo; Wu, Lian; Guo, Fei; Gu, Jiali; Tang, Xiaoling; Jiang, Ling; Liu, Ji; Zhou, Jiahai; Yu, Hongwei


Book ID
120570032
Publisher
Springer
Year
2012
Tongue
English
Weight
627 KB
Volume
97
Category
Article
ISSN
1432-0614

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Directed Evolution of an Esterase from P
✍ Henke, E.; Bornscheuer, U. T. πŸ“‚ Article πŸ“… 1999 πŸ› Walter de Gruyter GmbH & Co. KG 🌐 English βš– 165 KB

The gene encoding an esterase from Pseudomonas fluorescens (PFE) was subjected to random mutagenesis by error-prone PCR or by using the mutator strain Epicurian coli XL1-Red. Enzyme libraries were then created in microtiter plates by expression of PFE-variants in E. coli. These were assayed for impr