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Enhanced chemiluminescent assays for acetylcholine

✍ Scribed by Ternaux, Jean-Pierre ;Chamoin, Marie-Claude

Publisher
John Wiley and Sons
Year
1994
Weight
510 KB
Volume
9
Category
Article
ISSN
0884-3996

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✦ Synopsis

Acetylcholine and choline chemiluminescent assays have limitations when these compounds are detected in small areas of mammalian nervous tissue. Use of 7-dimethylaminonaphthalene-1.2-dicarbonic acid hydrazide (7-DMAN), instead of luminol, gives a threefold increase in emitted light in the chemiluminescent assay for acetylcholine based on the coupled choline oxidase-peroxidase reaction. Addition of light enhancers, such as para-iodophenol or D-luciferin, to luminol or 7-DMAN further increased the light emission. Under these conditions the detection limit for acetylcholine was 650 femtomoles. This enhanced chemiluminescent assay should be convenient for the detection of in vivo and in virro acetylcholine release from mammalian neurons.

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