𝔖 Bobbio Scriptorium
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Energy-Tunable Quantitative Hybridization Assay

✍ Scribed by Braunlin, William; Völker, Jens; Plum, Eric; Breslauer, Kenneth J.


Book ID
122137820
Publisher
Biophysical Society
Year
2013
Tongue
English
Weight
36 KB
Volume
104
Category
Article
ISSN
0006-3495

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The presence of allelic sequence variations in DNA fragments can be easily detected by measuring the extent of DNA strand exchange between test double-stranded PCR products (target) and labeled standard double-stranded PCR products (probe). Under selected hybridization conditions, sequences identica