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A solution hybridization assay for the quantitation of prodynorphin mRNA

✍ Scribed by Devi, Lakshmi; Douglass, James; Herbert, Edward

Book ID
123051874
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
231 KB
Volume
2
Category
Article
ISSN
0169-328X

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✍ Diane M. Durnam; Richard D. Palmiter πŸ“‚ Article πŸ“… 1983 πŸ› Elsevier Science 🌐 English βš– 752 KB

The preparation and use of a specific cDNA probe for quantitating mRNA by solution hybridization is described. Cloned DNA sequences are nick translated, denatured, hybridized to single-stranded M 13 clones containing message strand (mDNA) sequences, and separated chromatographically on Bio-Gel A50 u