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Endoplasmic reticulum protein expression in recombinant NS0 myelomas grown in batch culture

✍ Scribed by Matthew R. Downham; William E. Farrell; Helen A. Jenkins

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 634 KB
Volume: 51
Category: Article
ISSN: 0006-3592
DOI: 10.1002/(sici)1097-0290(19960920)51:6<691::aid-bit7>3.0.co;2-c

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✦ Synopsis

During batch growth of two recombinant NSO myelomas, an increase in the expression of the endoplasmic reticulum (ER) proteins (GRP78/BiP, GRP94, and ERp72) was observed. A marked increase in these proteinswas associated with the decline phase of growth, an increase in the production rate of chimeric antibody, and a marked slowing or halt in the uptake of glucose and glutamate. Refeeding with glucose, glutamate, or a mixture of amino acids just prior to the onset of decline phase failed to repress induction. Although refeeding with glutamate led to an increase in specific productivity, there was no significant difference in the pattern of ER protein induction. These results indicate that an increase in ER protein expression is not solely related to productivity but also to certain changes that occur during the course of batch growth. 0

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