Endogenous γ-L-glutamyl and β-L-aspartyl peptides and excitatory aminoacidergic neurotransmission in the brain
✍ Scribed by V Varga; R Janáky; P Saransaari; S.S Oja
- Book ID
- 113237284
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 772 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0143-4179
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📜 SIMILAR VOLUMES
## Abstract The separation and resolution of the isopeptides __N__ϵ(γ‐glutamyl)‐L‐lysine and __N__ϵ(β‐aspartyl)‐L‐lysine formed in heated proteins has been successfully achieved. The method demands a well‐characterised ion‐exchange column and the use of pH 3.40 lithium citrate buffer (0.2 N Li^+^).
A fast atom bombardment/collisional activation/linked-scan at constant B/E (tandem mass spectrometric) method is described which can distinguish between a-and basparty1 and a-and y-glutamyl underivatized peptides. The method is based upon differences in loss of CO from aspartyl or glutamyl B-fragmen