Endogenous porphyrin accumulation and photosensitization in the yeast Saccharomyces cerevisiae in the presence of 2,2′-dipyridyl

✍ Scribed by M.G. Strakhovskaya; A.O. Shumarina; G.Ya. Fraikin; A.B. Rubin

• Publisher: Elsevier Science
• Year: 1999
• Tongue: English
• Weight: 447 KB
• Volume: 49
• Category: Article
• ISSN: 1011-1344
• DOI: 10.1016/s1011-1344(98)00215-2

✦ Synopsis

The chelator 2,2'-dipyridyl (0.2 mM) induces a remarkable increase of protoporphyrin IX concentration as well as of its Zn-containing complex in the yeast Saccharomyces cerevisiae. Endogenous porphyrin accumulation results in five- to six-fold cell sensitization to visible light (400-600 nm). Mitochondria isolated from the cells grown in the presence of 2,2'-dipyridyl accumulate protoporphyrin IX and Zn-protoporphyrin IX, while plasma membranes besides that exhibit porphyrin-type fluorescence at 670-675 nm in chloroform extract. The protoporphyrin IX content increases more than four-fold in mitochondria and two-fold in plasma membranes isolated from chelator-treated cells. The relative contribution of subcellular structure photodestruction to photoinduced cell inactivation is discussed.