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Endogenous peroxidase in the visceral endoderm of early mouse embryos

✍ Scribed by Bałakier, Hanna

Publisher
John Wiley and Sons
Year
1984
Tongue
English
Weight
607 KB
Volume
231
Category
Article
ISSN
0022-104X

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✦ Synopsis

Endogenous peroxidase activity was demonstrated in early mouse embryos by means of the diaminobenzidine staining reaction. This enzyme was observed in visceral endoderm on the seventh to eighth day of gestation in vivo, but was no longer detected on the ninth day of development. In cell layers developing from blastocysts or isolated inner cell masses cultured for 96-144 h (developmental stage equivalent to 6-7.5-day-old embryos), diaminobenzidine product was also observed in visceral endodermal cells. Most of the endogenous peroxidase was localized inside or close to the numerous apical vacuoles in the endoderm. Ectoderm, mesoderm, ectoplacental cone, and trophoblast cells did not contain endogenous peroxidase.

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