End-labeled free-solution electrophoresis of DNA
β Scribed by Robert J. Meagher; Jong-In Won; Laurette C. McCormick; Sorin Nedelcu; Martin M. Bertrand; Jordan L. Bertram; Guy Drouin; Annelise E. Barron; Gary W. Slater
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 513 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0173-0835
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## Abstract In endβlabeled freeβsolution electrophoresis (ELFSE), DNA molecules are labeled with a frictional modifier or βdragβtagβ, allowing their sizeβbased electrophoretic separation in free solution. Among the interesting observations from early work with dsDNA using streptavidin as a dragβtag
## Diffusion coefficient of DNA molecules during free solution electrophoresis The free-draining properties of DNA normally make it impossible to separate nucleic acids by free-flow electrophoresis. However, little is known, either theoretically or experimentally, about the diffusion coefficient o