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End-labeled free-solution electrophoresis of DNA

✍ Scribed by Robert J. Meagher; Jong-In Won; Laurette C. McCormick; Sorin Nedelcu; Martin M. Bertrand; Jordan L. Bertram; Guy Drouin; Annelise E. Barron; Gary W. Slater


Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
513 KB
Volume
26
Category
Article
ISSN
0173-0835

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## Abstract In end‐labeled free‐solution electrophoresis (ELFSE), DNA molecules are labeled with a frictional modifier or β€œdrag‐tag”, allowing their size‐based electrophoretic separation in free solution. Among the interesting observations from early work with dsDNA using streptavidin as a drag‐tag

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## Diffusion coefficient of DNA molecules during free solution electrophoresis The free-draining properties of DNA normally make it impossible to separate nucleic acids by free-flow electrophoresis. However, little is known, either theoretically or experimentally, about the diffusion coefficient o