✦ LIBER ✦

Diffusion coefficient of DNA molecules during free solution electrophoresis

✍ Scribed by Axel E. Nkodo; Jean M. Garnier; Bernard Tinland; Hongji Ren; Claude Desruisseaux; Laurette C. McCormick; Guy Drouin; Gary W. Slater

Publisher: John Wiley and Sons
Year: 2001
Tongue: English
Weight: 130 KB
Volume: 22
Category: Article
ISSN: 0173-0835
DOI: 10.1002/1522-2683(200107)22:12<2424::aid-elps2424>3.0.co;2-1

No coin nor oath required. For personal study only.

✦ Synopsis

Diffusion coefficient of DNA molecules during free solution electrophoresis

The free-draining properties of DNA normally make it impossible to separate nucleic acids by free-flow electrophoresis. However, little is known, either theoretically or experimentally, about the diffusion coefficient of DNA molecules during free-flow electrophoresis. In fact, many authors simply assume that the Nernst-Einstein relation between the mobility and the diffusion coefficient still holds under such conditions. In this paper, we present an experimental study of the diffusion coefficient of both ssDNA and dsDNA molecules during free-flow electrophoresis. Our results unequivocally show that a simplistic use of Nernst-Einstein's relation fails, and that the electric field actually has no effect on the thermal diffusion process. Finally, we compare the dependence of the diffusion coefficient upon DNA molecular size to results obtained previously by other groups and to Zimm's theory.

📜 SIMILAR VOLUMES

Determining the electrophoretic mobility

Determining the electrophoretic mobility and translational diffusion coefficients of DNA molecules in free solution

✍ Earle Stellwagen; Nancy C. Stellwagen 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 149 KB 👁 2 views

The free solution mobility of DNA molecules of different molecular weights, the sequence dependence of the mobility, and the diffusion coefficients of small single- and double-stranded DNA (ss- and dsDNA) molecules can be measured accurately by capillary zone electrophoresis, using coated capillarie

Measuring the translational diffusion co

Measuring the translational diffusion coefficients of small DNA molecules by capillary electrophoresis

✍ Nancy C. Stellwagen; Soffia Magnusdottir; Cecilia Gelfi; Pier Giorgio Righetti 📂 Article 📅 2001 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 99 KB 👁 2 views

Simultaneous measurements of the electro

Simultaneous measurements of the electrophoretic mobility, diffusion coefficient and orientation of dsDNA during electrophoresis in polymer solutions

✍ Axel Ekani Nkodo; Bernard Tinland 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 177 KB 👁 2 views

We determined simultaneously the electrophoretic mobility, diffusion coefficient D and molecular orientation during electrophoresis of dsDNAs in polymer solutions ranging from the dilute to the semidilute regime. We established, for the first time, master scaling laws for the diffusion coefficient s

Capillary electrophoresis of small solut

Capillary electrophoresis of small solutes in linear polymer solutions: Relation between ionic mobility, diffusion coefficient and viscosity

✍ Tamo Shimizu; Ernst Kenndler 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 225 KB 👁 2 views

Influence of λ-DNA concentration on mobi

Influence of λ-DNA concentration on mobilities and dispersion coefficients during agarose gel electrophoresis

✍ Nadine Pernodet; Bernard Tinland 📂 Article 📅 1997 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 118 KB 👁 1 views

experimentally that during gel electrophoresis the mobility m and the dispersion coefficient D x [reflecting band broadening; G. W.

Estimation of an effective water diffusi

Estimation of an effective water diffusion coefficient during infrared-convective drying of a polymer solution

✍ N. Allanic; P. Salagnac; P. Glouannec; B. Guerrier 📂 Article 📅 2009 🏛 American Institute of Chemical Engineers 🌐 English ⚖ 452 KB 👁 1 views

## Abstract This article deals with the drying of an aqueous solution of polyvinyl alcohol mixed with a plasticizer. A heating combining forced convection and short‐infrared radiation was investigated. A one‐dimensional model taking into account the shrinkage of the product was developed to get the