Enantioselective separation of the carfentrazone-ethyl enantiomers in soil, water and wheat by HPLC

✍ Scribed by Yuanbo Li; Fengshou Dong; Xingang Liu; Jun Xu; Wuying Chen; Li Cheng; Ping Ning; Jing Li; Yunhao Wang; Yongquan Zheng

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 215 KB
• Volume: 33
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200900881

✦ Synopsis

Abstract

A simple enantioselective HPLC method was developed for measuring carfentrazone‐ethyl enantiomers. The separation and determination was accomplished on an amylose tris[(S)‐α‐methylbenzylcarbamate] (Chiralpak AS) column using n‐hexane/ethanol (98:2, v/v) as mobile phase at a flow rate of 1.0 mL/min with UV detection at 248 nm. The effects of mobile‐phase composition and column temperature on the enantioseparation were discussed. The accuracy, precision, linearity, LODs, and LOQ of the method were also investigated. LOD was 0.001 mg/kg in water, 0.015 mg/kg in soil and wheat, with an LOQ of 0.0025 mg/kg in water and 0.05 mg/kg in soil and wheat for each enantiomer of carfentrazone‐ethyl. SPE was used for the enrichment and cleanup of soil, water, and wheat samples. Recoveries for two enantiomers were 88.4–106.7% with RSD~r~ of 4.2–9.8% at 0.1, 0.5, and 1 mg/kg levels from soil, 85.8–99.5% with the RSD~r~ of 4.4−9.6% at 0.005, 0.025, and 0.05 mg/kg levels from water, and from wheat the recoveries were 86.3−91.3% with RSD~r~ below 5.0% at 0.2, 0.5, and 1 mg/kg levels. This method could be used to identify and quantify the carfentrazone‐ethyl enantiomers in food and environment.