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Enantioselective determination of bromoisovalerylurea by liquid chromatography on chiral stationary phase in reversed- or normal-phase partition mode

✍ Scribed by Takashi Nishikawa; Hideki Ohtani; Yoshito Kamijo; Yoshio Ohtani; Rumiko Kondo; Hisako Takeuchi; Takanori Okuda

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 76 KB
Volume: 14
Category: Article
ISSN: 0269-3879
DOI: 10.1002/1099-0801(200006)14:4<243::aid-bmc977>3.0.co;2-i

No coin nor oath required. For personal study only.

✦ Synopsis

Bromoisovalerylurea (bromvalerylurea) is a sedative-hypnotic given orally as a racemate. Enantiomers of this drug could be separated by high-performance liquid chromatography on the three chiral stationary phases (a vancomycin-bonded, b-cyclodextrin derivative-bonded, or urea derivative-bonded phase). Biological fluids of human subjects who had ingested toxic or therapeutic doses of the racemate were chromatographed after liquid-liquid extraction. The ()-enantiomer concentration was almost equal to the (À)-enantiomer concentration in the serum of one overdosed patient. In all the other subjects, the ()-enantiomer was less than the (À)-enantiomer in their sera and saliva. The data suggest that the drug is absorbed non-stereoselectively from the gastrointestinal tract and eliminated from the blood stereoselectively.

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