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Enantiomer separation using BSA as chiral stationary phase in affinity OTEC and OTLC

✍ Scribed by Heike Hofstetter; Oliver Hofstetter; Volker Schurig

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 97 KB
Volume: 10
Category: Article
ISSN: 1040-7685
DOI: 10.1002/(sici)1520-667x(1998)10:3<287::aid-mcs8>3.0.co;2-c

No coin nor oath required. For personal study only.

✦ Synopsis

The protein bovine serum albumin BSA was chemically bound to the inner surface of fused silica capillaries by a gentle coupling procedure in order to form a stable chiral stationary phase. Enantiomer separation of a number of DNP-amino acids and 3-hydroxy-1,4-benzodiazepines was possible in affinity open Ž . tubular electrokinetic chromatography affinity OTEC and open tubular liquid Ž . chromatography OTLC in one instrumental setup. This approach combines the advantages of high efficiency and small consumption of solvent and chiral selector with the possibility of UV detection without limitations of protein absorption.

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