Enantiomer separations of phenylephrine and synephrine by capillary electrochromatography on bare silica stationary phase using hydroxypropyl-β-cyclodextrin as a mobile phase additive
✍ Scribed by Wei Wei; Guoan Luo; Rong Xiang; Chao Yan
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 139 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1040-7685
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✦ Synopsis
The feasibility of enantiomer separation by capillary electrochromatog-Ž . Ž . raphy CEC was shown on 75-m i.d. capillary columns packed with 3-m bare w Ž . silica packings. Racemic phenylephrine 3-hydroxy-␣methylaminomethyl benzyl x w Ž .
x alconhol and synephrine 4-hydroxy-␣-methylaminomethyl benzyl alconhol were resolved on bare silica stationary phase using hydroxypropyl--cyclodextrin Ž . HP CD as a chiral mobile phase additive. The effects of HP CD concentration, pH of mobile phase, electrolyte concentration, and separation temperature have been investigated. Optimum separations were achieved for phenylephrine using 10 Ž . mM Tris᎐H PO buffer pH 3.12 containing 14 mM HP CD and those of 3 4
synephrine with HP CD concentration 18 mM at 25ЊC. The chiral recognition mechanisms in these systems were also discussed.