Enantiomer separation of DNP-amino acids by capillary electrophoresis using chiral buffer additives

✍ Scribed by Dorothee Wistuba; Heike Diebold; Volker Schurig

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 426 KB
• Volume: 7
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/mcs.1220070103

✦ Synopsis

The direct enantiomeric analysis of six DNP-amino acids by capillary electrophoresis is reported. This study describes a comparison of several chiral buffer additives. Both the natural proteins BSA, AGP, ovomucoid and casein, and the cyclic oligosaccharides a-, O-, and y-cyclodextrin were used as chiral selectors. For the first time, casein from goat milk was successfully applied for chiral discrimination. The influence of the modifier n-propanol on chiral discrimination is demonstrated with BSA as a buffer additive.