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Enantiomer separation of amino acids in immunoaffinity micro LC-MS

✍ Scribed by Jessica M. Zeleke; Gregory B. Smith; Heike Hofstetter; Oliver Hofstetter

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
138 KB
Volume
18
Category
Article
ISSN
0899-0042

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✦ Synopsis

Abstract

Chiral immunoaffinity microbore columns were directly interfaced with MS detection, and the effect of column length and temperature on the enantiomer separation of a number of underivatized aromatic and aliphatic amino acids was investigated utilizing an antibody chiral stationary phase that had been prepared by immobilizing a monoclonal anti‐D‐amino acid antibody onto silica. The stronger affinity of the antibody towards aromatic and bulky amino acids allowed separation of such analytes in a 0.75 × 150 mm column, while an increase in column length enabled separation of more weakly bound compounds. The strength of interaction between chiral selector and analytes could be modulated conveniently by lowering the temperature. For the first time, simultaneous enantiomer separation of mixtures of amino acids was achieved on antibody‐based chiral stationary phases using extracted ion chromatograms. Chirality 18:544–550, 2006. © 2006 Wiley‐Liss, Inc.

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