Fast electrophoretic separation of sulfur- and selenium-containig amino acid enantiomers with vancomycin as a chiral selector in coated capillaries
✍ Scribed by Vespalec, Radim ;Billiet, Hugo A. H. ;Frank, Johannes ;Luyben, Karel Ch. A. M.
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 438 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0935-6304
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✦ Synopsis
Abstract
Baseline separation of the enantiomers of the negatively charged 6‐aminoquinolyl‐N‐hydroxysuccinimidyl carbamate (AQC) derivatives of cystine, methionine, ethionine, and their seleno analogs can be achieved in 3–5 min with capillary electrophoresis in polyacrylamide coated capillaries and submillimolar concentrations of vancomycin as the chiral selector.
In addition to the vancomycin concentration, the separation is affected by the type, concentration and pH of the buffer. Good buffers are more suitable than phosphate buffer. At pH values above the isoelectric point of vancomycin the mobility difference between the enantiomers becomes smaller. This effect is larger than would be expected from the reversal of the vancomycin migration alone.