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Elimination of thiol reagent interference during Lowry protein determination

✍ Scribed by James Hughes; Saroj Joshi; Diane Ascoli

Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
394 KB
Volume
117
Category
Article
ISSN
0003-2697

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✦ Synopsis

Pretreatment of protein samples containing mono-or dithiol reagents with N-ethylmaleimide significantly reduces interference during subsequent protein determinations by the Lowry method. When the Lowry procedure as modified by M. A. K. Markwell er al. (Anal. Biochem. 87, 206) is utilized, the effectiveness of N-ethylmaleimide pretreatment is not influenced by a variety of substances including detergents, lipids, EDTA, sucrose, and salts. The combination of the modified procedure of Markwell et al. with N-ethylmaleimide pretreatment is rapid, easily applied to a large number of samples, and equally applicable to both soluble and membranous protein samples.

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Polyacrylamide gel interference of the l
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✍ P.A. Fields; L.H. Larkin πŸ“‚ Article πŸ“… 1978 πŸ› Elsevier Science 🌐 English βš– 406 KB

Certain reagents utilized in the formation of polyacrylamide gels are shown to interfere in the Lowry assay for protein. Acrylamide (3-30%) and potassium ferrocyanide (O.OOlS-0.0105%) produced a linear response in color formation. Both compounds are capable of reducing the phenol reagent in the abse