Elimination of adsorption effects in gel permeation chromatography of humic substances

✍ Scribed by Raewyn M. Town; H. Kipton; J. Powell

• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 505 KB
• Volume: 256
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(92)85330-9

✦ Synopsis

Elutlon of humlc substances

from Sephadex G-100 gel was optlmlzed by use of samples filtered through a 0 025qm membrane filter and 0 01 mol 1-l dlsodmm tetraborate eluent However, some material was stdl retamed on the column, probably through complexmg to metal Ions bound to the gel This problem was eliminated by addition of 0 001 mol 1-l Na,P,O, to the eluent