Elemental mass spectrometry for quantitative proteomics
✍ Scribed by Alfredo Sanz-Medel; María Montes-Bayón; María del Rosario Fernández de la Campa; Jorge Ruiz Encinar; Jörg Bettmer
- Publisher
- Springer
- Year
- 2007
- Tongue
- English
- Weight
- 351 KB
- Volume
- 390
- Category
- Article
- ISSN
- 1618-2650
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📜 SIMILAR VOLUMES
The use of stable isotopes as internal standards in mass spectrometry has opened a new era for quantitative proteomics. Depending on the point at which the label is introduced, most procedures can be classified as in vivo labeling, in vitro pre-digestion labeling or in vitro post-digestion labeling.
## Abstract | I. | Introduction | 182 | | II. | Relative Quantitation | 184 | | | A. 2‐D Gel Electrophoresis | 184 | | | B. Metabolic Isotopic Labeling | 185 | | | 1. ^15^N | 185 | | | 2. ^13^C Enrichment and Depletion | 185 | | | 3. Select Isotopic Amino Acid Incorporation