New Developments of Quantitative Mass Spectrometry-based Proteomics
β Scribed by Jin-Lei ZHU; Kai ZHANG; Xi-Wen HE; Yu-Kui ZHANG
- Publisher
- Elsevier Science
- Year
- 2010
- Tongue
- Chinese
- Weight
- 320 KB
- Volume
- 38
- Category
- Article
- ISSN
- 1872-2040
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π SIMILAR VOLUMES
The use of stable isotopes as internal standards in mass spectrometry has opened a new era for quantitative proteomics. Depending on the point at which the label is introduced, most procedures can be classified as in vivo labeling, in vitro pre-digestion labeling or in vitro post-digestion labeling.
## Abstract | ββI. | Introduction | 182 | | βII. | Relative Quantitation | 184 | | | A.ββ2βD Gel Electrophoresis | 184 | | | B.ββMetabolic Isotopic Labeling | 185 | | | ββββ1.ββ^15^N | 185 | | | ββββ2.ββ^13^C Enrichment and Depletion | 185 | | | ββββ3.ββSelect Isotopic Amino Acid Incorporation