Proteins are extracted from liver into aqueous buffer at pH 7 and separated by size exclusion chromatography (SEC). Inductively coupled plasma mass spectrometry (ICP-MS) with a magnetic sector mass spectrometer is used to identify those protein fractions that contain Cu,
Elemental analysis by inductively coupled plasma mass spectrometry with sector field instruments: a progress report
β Scribed by Stuewer, Dietmar; Jakubowski, Norbert
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 311 KB
- Volume
- 33
- Category
- Article
- ISSN
- 1076-5174
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β¦ Synopsis
Inductively coupled plasma mass spectrometry (ICP-MS) for elemental analysis has been dominated since its introduction by instruments with quadrupole Γlters for mass separation. Although considerably impeded by isobaric ion interferences, ICP-MS with quadrupoles has nevertheless matured to become the most prominent MS technique for element analysis with widespread applications, providing extremely low detection limits in combination with true multi-element capabilities. Some years ago, a second generation of double-focusing ICP-MS instruments was introduced, o β ering the chance to overcome interference problems by high mass resolution. The special features of these instruments for elemental analysis are discussed and the progress achieved in analytical performance is demonstrated by selected examples, emphasizing speciation as a Γeld in which organic and inorganic aspects come close together.
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