Electroporation of fish sperm for gene transfer
โ Scribed by SIN, F; BARTLEY, A; WALKER, S; BULMAN, S; ALLISON, L; HOPKINS, C; SIN, I
- Book ID
- 123412538
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 139 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0309-1651
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We investigated gene transfer in finfish and shellfish via electroporated sperm. The mobility of sperm, the fertilization rate, the hatching rate, gene transfer rate, and abnormality rate of derived embryos were primarily dependent on the voltage level and concentration of DNA during electroporation
Uptake of exogenous DNA by electroporated salmon sperm for gene transfer is being investigated. Our studies show that electroporated salmon sperm cells were more efficient and more reliable than untreated sperm in picking up exogenous DNA and subsequently transferring the DNA into salmon embryos. In