𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Electroporation of salmon sperm for gene transfer: Efficiency, reliability, and fate of transgene

✍ Scribed by F.Y.T. Sin; S.P. Walker; J.E. Symonds; U.K. Mukherjee; J.G.I. Khoo; I.L. Sin

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
145 KB
Volume
56
Category
Article
ISSN
1040-452X

No coin nor oath required. For personal study only.

✦ Synopsis

Uptake of exogenous DNA by electroporated salmon sperm for gene transfer is being investigated. Our studies show that electroporated salmon sperm cells were more efficient and more reliable than untreated sperm in picking up exogenous DNA and subsequently transferring the DNA into salmon embryos. Indirect evidence suggest that some of the exogenous DNA was internalized in the sperm nuclei. The taken up DNA retained its integrity as demonstrated by PCR. The foreign DNA was detected in 15-month-old fish, and had a mosaic pattern of distribution. Integration of the foreign DNA occurred infrequently, and the expression of the foreign genes was poor. The potential of sperm-mediated gene transfer as a routine protocol for mass gene transfer in salmon will be dependent on the improvement of integration and expression of the foreign gene.

📜 SIMILAR VOLUMES

Electroporated sperm mediation of a gene
Electroporated sperm mediation of a gene transfer system for finfish and shellfish
✍ Huai-Jen Tsai 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 98 KB

We investigated gene transfer in finfish and shellfish via electroporated sperm. The mobility of sperm, the fertilization rate, the hatching rate, gene transfer rate, and abnormality rate of derived embryos were primarily dependent on the voltage level and concentration of DNA during electroporation

Retrovirus-mediated transfer of the herp
Retrovirus-mediated transfer of the herpes simplex virus thymidine kinase and connexin26 genes in pancreatic cells results in variable efficiency on the bystander killing: Implications for gene therapy
✍ Meritxell Carrió; Adela Mazo; Carmen López-Iglesias; Xavier Estivill; Cristina F 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 French ⚖ 394 KB

Currently, there is no effective treatment for pancreatic cancer and prodrug-activating gene therapy with the herpes simplex virus thymidine kinase gene (HSV-tk) in combination with ganciclovir (GCV) has been suggested as a candidate approach against this disease. In the present study, we have evalu

Baculovirus is an efficient vector for t
Baculovirus is an efficient vector for the transduction of the eye: comparison of baculovirus- and adenovirus-mediated intravitreal vascular endothelial growth factor D gene transfer in the rabbit eye
✍ Kati Kinnunen; Giedrius Kalesnykas; Anssi J. Mähönen; Svetlana Laidinen; Liisa H 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 348 KB

## Abstract ## Background The present study aimed to determine the efficiency and safety of baculovirus‐mediated intravitreal gene transfer in rabbit eye and to compare its efficiency with adenovirus. We also studied how an intravitreal injection of vectors producing vascular endothelial growth fa